The KBS Molecular Ecology and Genomics (MEG) Lab is a state of the art xf187 PT安装版research and teaching laboratory established in 2017 to support the growing research and teaching conducted at KBS that involves molecular tools and bioinformatic analyses.This shared facility supports a diverse set of Next-Generation-Sequencing (NGS) pipelines,from DNA/RNA extraction through library preparation and sample send-off.最终目标是推进将“OMIC”研究与基于实地的自然历史和长期实地研xf187 PT安装版究结合起来的研究。
The MEG lab is widely used by resident and visiting faculty,学生，and xf187 PT安装版research associates.工作台空间，consumables (tips,管，gloves) and instrumentation is available to visitors for a small fee.接触Sarah Fitzpatrickfor more information.
The MEG lab is equipped with the full range of molecular xf187 PT安装版research equipment necessary for DNA and RNA extraction,聚合酶链反应凝胶电泳，and various genomic library preparation protocols.We have an Apple iMac dedicated to bioinformatics and working with molecular data and have access to Michigan State University'sHigh Performance Computing Cluster.In addition to standard equipment (centrifuges,-20和-80冷冻机，吸管，thermocyclers) we have the following specialized equipment available for use:
- Agilent 2100 Bioanalyzer
- NuAire Class II,B2型生物安全柜
- Covaris M220 Focused-ultrasonicator
- Eppendorf liquid handling robot
- Kingfisher Flex Purification System
- Milli-Q Integral water purification system
- Qubit3 fluorometer
- Qiagen Tissue Lyser
Conducting xf187 PT安装版research at the MEG Lab
Fee structure TBD
xf187 PT安装版Research highlights
Fitness effects of loss-of-function mutations in duplicate genes –Conner Lab in collaboration withShin-Han Shiu (MSU) and Patrick Krysan (Wisconsin)；基金资助
Origins and adaptations of weedy and crop radish –Conner Lab in collaboration with Kevin Childs and Patrick Edger (MSU);拉克姆基金会资助
Microbial ecology and litter decomposition in a hyperarid desert –Evans lab in collaboration with Kathy and Peter Jacobson (Grinnell College);funded by Michigan State University and National Geographic Society.
Connecting nitrogen transformations mediated by the rhizosphere microbiome to perennial cropping system productivity in marginal lands.Evans Lab in collaboration with Lisa Tiemann,Jim Cole（MSU），Maren Friesen (Washington State University);能源部资助
Range-wide landscape genomics of a threatened species of Great Plains fish –Fitzpatrick Lab;funded by US Fish & Wildlife and Kansas Dept.of Parks & Tourism
Determining QTL underlying behavioral variation in Trinidadian guppies –Fitzpatrick Lab in collaboration with Kim Hughes (FSU) & Kim Hoke (CSU);基金资助
伊万斯东南方，我Dueker，小洛根，KC Weathers (2019) The biology of fog: results from coastal Maine and Namib Desert reveal common drivers of fog microbial composition.Science of The Total Environment64 7:1544-1556链接
菲茨帕特里克SWBN瑞德在审查中) Does gene flow aggravate or alleviate maladaptation to environmental stress in small populations?
菲茨帕特里克SWG BradburdC Kremer萨勒诺LM AngeloniWC Funk在审查中) Genetic rescue without genomic swamping in replicate Trinidadian guppy populations in the wild